Anti-FGF5 antibody (ab88118)
Key features and details
- Rabbit polyclonal to FGF5
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FGF5 antibody
See all FGF5 primary antibodies -
Description
Rabbit polyclonal to FGF5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human FGF5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab106210) -
Positive control
- Recombinant human FGF5 protein (ab50229) can be used as a positive control in WB. This anitbody gave a positive signal in the following tissues lysates: E14 Mouse Embryo Brain; E16 Ms Embryo Brain; E18 Ms Embryo Brain ICC/IF: NIH3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase. -
Tissue specificity
Expressed in neonatal brain. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Developmental stage
Can transform NIH 3T3 cells. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 536771 Cow
- Entrez Gene: 2250 Human
- Entrez Gene: 14176 Mouse
- Omim: 165190 Human
- SwissProt: A0MTF4 Cow
- SwissProt: P12034 Human
- SwissProt: P15656 Mouse
- Unigene: 37055 Human
see all -
Alternative names
- FGF 5 antibody
- FGF-5 antibody
- FGF5 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FGF5 antibody (ab88118)ab88118 staining FGF5 in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab88118 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-FGF5 antibody (ab88118)All lanes : Anti-FGF5 antibody (ab88118) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E16 Ms Embryo Brain Tissue Lysate
Lane 3 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab88118 has been referenced in 5 publications.
- Dai Y et al. Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C. Cell Death Discov 8:344 (2022). PubMed: 35915078
- Vila-Cejudo M et al. Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features. J Assist Reprod Genet 37:2967-2979 (2020). PubMed: 33047186
- Chen B et al. FGF5 Regulates Schwann Cell Migration and Adhesion. Front Cell Neurosci 14:237 (2020). PubMed: 32848626
- Dai Y et al. Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. Am J Transl Res 12:8147-8161 (2020). PubMed: 33437388
- Ding BS et al. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci 128:2983-8 (2015). IHC ; Human . PubMed: 26116571
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FGF5 antibody (ab88118)
ICC/IF image of ab88118 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab88118 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-FGF5 antibody (ab88118)All lanes : Anti-FGF5 antibody (ab88118) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E16 Ms Embryo Brain Tissue Lysate
Lane 3 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
