Anti-FAM96B antibody (ab230410)
Key features and details
- Rabbit polyclonal to FAM96B
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FAM96B antibody
See all FAM96B primary antibodies -
Description
Rabbit polyclonal to FAM96B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human FAM96B aa 1 to the C-terminus.
Database link: Q9Y3D0 -
Positive control
- WB: Mouse gonadal tissue; MCF7, HeLa, K562 and A375 whole cell lysates. IHC-P: Human kidney tissue. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAM96B antibody (ab230410)All lanes : Anti-TRIM10 antibody (ab230409) at 1/10000 dilution
Lane 1 : Mouse gonadal tissue lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 18 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM96B antibody (ab230410)Paraffin-embedded human kidney tissue stained for FAM96B using ab230410 at 1/100 dilution in immunochemical analysis.
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Immunoprecipitation - Anti-FAM96B antibody (ab230410)FAM96B was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg of ab230410. Western blot was performed from the immunoprecipitate using ab230410 and an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50,000 dilution.
Lane 1: 1 µg of rabbit monoclonal IgG instead of ab230410 in HeLa whole cell lysate.
Lane 2: ab230410 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (input).
