Anti-PLD2 (phospho Y169) antibody (ab78907)
Key features and details
- Rabbit polyclonal to PLD2 (phospho Y169)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLD2 (phospho Y169) antibody
See all PLD2 primary antibodies -
Description
Rabbit polyclonal to PLD2 (phospho Y169) -
Host species
Rabbit -
Specificity
ab78907 detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
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Positive control
- IHC-P: human brain tissue WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab78907 was purified by affinity chromatography using an epitope specific phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PLD2 (phospho Y169) antibody (ab78907)All lanes : Anti-PLD2 (phospho Y169) antibody (ab78907) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
Lane 2 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 106 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLD2 (phospho Y169) antibody (ab78907)Immunohistochemical analysis of PLD2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.
