ChIP Kit (FFPE samples) (ab270815)
Overview
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Product name
ChIP Kit (FFPE samples) -
Product overview
ChIP Kit (FFPE samples) (ab270815) provides a unique and robust ChIP protocol suitable for investigation of proteins associated with chromatin from FFPE samples and provides high quality yields of DNA. This solution makes traditionally difficult work with FFPE samples simpler and safer. The protocol involves easy and quick and non-toxic deparaffinization, followed by chromatin extraction and shearing by sonication. The kit contains everything you need for start-to-finish chromatin immunoprecipitation with an optimized protocol to make ChIP from FFPE samples simple yet reliable. In addition, the kit has been thoroughly validated and optimized for histones and transcription factors.
This kit contains enough reagents to perform 10 individual chromatin preparation from FFPE sections, up to 24 immunoprecipitations and 50 DNA purifications (from 10 input, 24 IPs and 10 shearing assessment samples).
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Please refer to protocols. -
Components 24 tests 5% BSA (DNA free) 1 x 144µl 5x ChIP Buffer C1b 1 x 6.8ml ChIP DNA Binding Buffer 1 x 50ml ChIP-seq grade water 1 x 26.6ml Filters 10 units Protein A-coated magnetic beads 1 x 480µl DNA Elution Buffer 1 x 10ml DNA Wash Buffer 1 x 6ml Elution Buffer E1 1 x 7.8ml Elution Buffer E2 1 x 320µl Lysis Buffer L1b 1 x 6ml Lysis Buffer L2 1 x 6ml Protease inhibitor cocktail 1 x 80µl Shearing Buffer S1b 1 x 6ml Spin columns 50 units Wash buffer W1 1 x 8.4ml Wash buffer W2 1 x 8.4ml Wash buffer W3 1 x 8.4ml Wash Buffer W4 1 x 8.4ml
