Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S2P antibody (ab140594)

Key features and details

  • Rabbit polyclonal to S2P
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-S2P antibody
    See all S2P primary antibodies

  • Description

    Rabbit polyclonal to S2P

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human S2P aa 300-400 conjugated to keyhole limpet haemocyanin.
    Database link: O43462

  • Positive control

    • This antibody gave a positive signal in Human Heart and Liver tissue lysates.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab140594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Intramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop.

  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

  • Involvement in disease

    Defects in MBTPS2 are the cause of ichthyosis follicularis-atrichia-photophobia syndrome (IFAPS) [MIM:308205]. A syndrome characterized by a peculiar triad of follicular ichthyosis, total or subtotal atrichia, and photophobia of varying degree. Histopathologically, the epidermal granular layer is generally well-preserved or thickened at the infundibulum. Hair follicles are poorly developed and tend to be surrounded by an inflammatory infiltrate. A subgroup of patients is described with lamellar rather than follicular ichthyosis. Non-consistent features may include growth and psychomotor retardation, aganglionic megacolon, seizures and nail dystrophy.
    Defects in MBTPS2 are a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX) [MIM:308800]. A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration.

  • Sequence similarities

    Belongs to the peptidase M50A family.

  • Cellular localization

    Membrane. Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BRESEK antibody
    • Endopeptidase S2P antibody
    • IFAP antibody
    • KFSDX antibody
    • MBTP2_HUMAN antibody
    • MBTPS2 antibody
    • Membrane bound transcription factor site 2 protease antibody
    • membrane-bound transcription factor peptidase, site 2 antibody
    • membrane-bound transcription factor protease, site 2 antibody
    • Membrane-bound transcription factor site-2 protease antibody
    • S2P antibody
    • S2P endopeptidase antibody
    • Site 2 protease antibody
    • SREBPs intramembrane protease antibody
    • Sterol regulatory element-binding proteins intramembrane protease antibody
    see all

Images

  • Western blot - Anti-S2P antibody (ab140594)
    Western blot - Anti-S2P antibody (ab140594)
    All lanes : Anti-S2P antibody (ab140594) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 26 kDa (possible non-specific binding), 40 kDa (possible non-specific binding), 62 kDa (possible glycosylated form)


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140594 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References (1)

Publishing research using ab140594? Please let us know so that we can cite the reference in this datasheet.

ab140594 has been referenced in 1 publication.

  • Guo C  et al. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. Immunity 49:842-856.e7 (2018). PubMed: 30366764

Images

  • Western blot - Anti-S2P antibody (ab140594)
    Western blot - Anti-S2P antibody (ab140594)
    All lanes : Anti-S2P antibody (ab140594) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 26 kDa (possible non-specific binding), 40 kDa (possible non-specific binding), 62 kDa (possible glycosylated form)


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140594 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

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