Anti-ERp29 antibody [EPR12985] - BSA and Azide free (ab249848)
![Anti-ERp29 antibody [EPR12985] - BSA and Azide free (ab249848)](https://www.abcam.com/ps/products/249/ab249848/Images/ab249848-384520-anti-erp29-antibody-epr12985-bsa-and-azide-free-western-blot-he293t-hepg2.jpg "Anti-ERp29 antibody [EPR12985] - BSA and Azide free (ab249848)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12985] to ERp29 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-ERp29 antibody [EPR12985] - BSA and Azide free
See all ERp29 primary antibodies -
Description
Rabbit monoclonal [EPR12985] to ERp29 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T cell lysate.
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General notes
ab249848 is the carrier-free version of ab175193 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249848 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12985 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERp29 antibody [EPR12985] - BSA and Azide free (ab249848)All lanes : Anti-ERp29 antibody [EPR12985] (ab175193) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ERP29 knockout HEK293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab175193).
Lanes 1-3: Merged signal (red and green). Green - ab175193 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab175193 Anti-ERp29 antibody [EPR12985] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab175193 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Anti-ERp29 antibody [EPR12985] - BSA and Azide free (ab249848)](https://www.abcam.com/ps/products/249/ab249848/Images/ab249848-2-benefits-of-recombinant-antibodies.png)
Anti-ERp29 antibody [EPR12985] - BSA and Azide free (ab249848)
