Recombinant human Thioredoxin / TRX protein (Active) (ab51064)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
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Product name
Recombinant human Thioredoxin / TRX protein (Active)
See all Thioredoxin / TRX proteins and peptides -
Biological activity
Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
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Purity
> 95 % SDS-PAGE.
Recombinant human Thioredoxin / TRX was overexpressed in E. coli and purified by using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV -
Predicted molecular weight
12 kDa -
Amino acids
1 to 105 -
Additional sequence information
(NP_003320)
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human Thioredoxin / TRX protein (ab51064)
3 ug of reduced ab51064, on 15% SDS-PAGE, stained with Coomassie Blue.
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Western blot - Recombinant human Thioredoxin / TRX protein (ab51064)Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml +Recombinant human Thioredoxin / TRX protein (Active) (ab51064) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
