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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases

Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)

Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP7235] to EPM2A/Laforin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free
    See all EPM2A/Laforin primary antibodies
  • Description

    Rabbit monoclonal [EP7235] to EPM2A/Laforin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): HeLa cells
  • General notes

    ab248308 is the carrier-free version of ab129110.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP7235
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Western blot - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    Western blot - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    All lanes : Anti-EPM2A/Laforin antibody [EP7235] (ab129110) at 1/100 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Human fetal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 37 kDa



    This data was developed using ab129110, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    This data was developed using ab129110, the same antibody clone in a different buffer formulation.ab129110, at 1/100 dilution, staining EPM2A/Laforin in Paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    Flow Cytometry - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    This data was developed using ab129110, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling EPM2A/Laforin with purified ab129110 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    This data was developed using ab129110, the same antibody clone in a different buffer formulation.ab129110, at 1/100 dilution, staining EPM2A/Laforin in Paraffin-embedded Human muscle tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    Immunocytochemistry - Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    This data was developed using ab129110, the same antibody clone in a different buffer formulation.ab129110, at 1/100 dilution, staining EPM2A/Laforin in HeLa cells by Immunofluorescence.
  • Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)
    Anti-EPM2A/Laforin antibody [EP7235] - BSA and Azide free (ab248308)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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