Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178)
![Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178)](https://www.abcam.com/ps/products/183/ab183178/Images/ab183178-412996-anti-epcam-antibody-e144-bsa-and-azide-free-western-blot-a431-wt-a431-epcam-knockout-mcf7-hela2.jpg "Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E144] to EpCAM - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-EpCAM antibody [E144] - BSA and Azide free
See all EpCAM primary antibodies -
Description
Rabbit monoclonal [E144] to EpCAM - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 and MCF7 cell lysates.
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General notes
Ab183178 is the carrier-free version of ab32392. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab183178 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E144 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178)All lanes : Anti-EpCAM antibody [E144] (ab32392) at 1/2000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : EPCAM knockout A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab32392).
Lanes 1 - 4: Merged signal (red and green). Green - ab32392 observed at 40 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab32392 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EpCAM knockout sample. Wild-type and EpCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab32392 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178)](https://www.abcam.com/ps/products/183/ab183178/Images/ab183178-405402-anti-epcam-antibody-e144-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178) -
![Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178)](https://www.abcam.com/ps/products/183/ab183178/Images/ab183178-3-benefits-of-recombinant-antibodies.png)
Anti-EpCAM antibody [E144] - BSA and Azide free (ab183178)
