Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Emi1 antibody [EPR15320] (ab184950)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15320] to Emi1
Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
Reacts with: Human

Product name

Anti-Emi1 antibody [EPR15320]
See all Emi1 primary antibodies
Description

Rabbit monoclonal [EPR15320] to Emi1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2 and HeLa cells and cell lysates; 293 and K562 cell lysates; Human ovarian carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15320
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] (ab184950)

Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody. The slide on the right is counter stained with Dapi (blue).
Western blot - Anti-Emi1 antibody [EPR15320] (ab184950)

All lanes : Anti-Emi1 antibody [EPR15320] (ab184950) at 1/5000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] (ab184950)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Emi1 with ab184950 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Emi1 antibody [EPR15320] (ab184950)

Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/180 dilution (red). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (green).
Anti-Emi1 antibody [EPR15320] (ab184950)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com