Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Emi1 antibody [EPR15320] (ab184950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15320] to Emi1
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Emi1 antibody [EPR15320]
    See all Emi1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15320] to Emi1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 and HeLa cells and cell lysates; 293 and K562 cell lysates; Human ovarian carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15320

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] (ab184950)
    Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] (ab184950)

    Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody. The slide on the right is counter stained with Dapi (blue).

  • Western blot - Anti-Emi1 antibody [EPR15320] (ab184950)
    Western blot - Anti-Emi1 antibody [EPR15320] (ab184950)
    All lanes : Anti-Emi1 antibody [EPR15320] (ab184950) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] (ab184950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] (ab184950)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Emi1 with ab184950 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Emi1 antibody [EPR15320] (ab184950)
    Flow Cytometry - Anti-Emi1 antibody [EPR15320] (ab184950)

    Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/180 dilution (red). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (green).

  • Anti-Emi1 antibody [EPR15320] (ab184950)
    Anti-Emi1 antibody [EPR15320] (ab184950)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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