Anti-Emi1 antibody [EMI1/1176] (ab215765)
Key features and details
- Mouse monoclonal [EMI1/1176] to Emi1
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Emi1 antibody [EMI1/1176]
See all Emi1 primary antibodies -
Description
Mouse monoclonal [EMI1/1176] to Emi1 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Emi1 aa 1-250. The exact sequence is proprietary. corresponding to a fragment of 203 amino acids.
Database link: Q9UKT4 -
Positive control
- HeLa, 293, K562 or HepG2 cell lysates. Ovarian carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
EMI1/1176 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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ab215765 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
All lanes : Anti-Emi1 antibody [EMI1/1176] (ab215765) at 2 µg/ml
Lane 1 : HeLa cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : HEK293 cell lysates
Lane 4 : K562 cell lysates
Predicted band size: 50 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (ab215765)
Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (ab215765)
Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.