Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Emi1 antibody [EPR15320-103] (ab187144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15320-103] to Emi1
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Emi1 antibody [EPR15320-103]
    See all Emi1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15320-103] to Emi1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293, HepG2 whole cell lysate (ab7900); Human ovarian carcinoma and mouse liver tissues; HeLa and HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15320-103

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320-103] (ab187144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320-103] (ab187144)

    Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Emi1 with ab187144 at 1/100 with a  prediluted HRP Polymer for Rabbit IgG as secondary antibody.

  • Western blot - Anti-Emi1 antibody [EPR15320-103] (ab187144)
    Western blot - Anti-Emi1 antibody [EPR15320-103] (ab187144)
    All lanes : Anti-Emi1 antibody [EPR15320-103] (ab187144) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation - Anti-Emi1 antibody [EPR15320-103] (ab187144)
    Immunoprecipitation - Anti-Emi1 antibody [EPR15320-103] (ab187144)

    Immunoprecipitation of Emi1 from HepG2 cell lysate using ab187144 at 1/50 (lane 1) or 1X PBS (negative control) (lane 2). Immunostained with ab187144 at 1/1000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320-103] (ab187144)
    Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320-103] (ab187144)

    Immunofluorescent analysis of HepG2 cells labeling Emi1 with ab187144 at 1/250 and DAPI staining (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320-103] (ab187144)
    Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320-103] (ab187144)

    Immunofluorescent analysis of HeLa cells labeling Emi1 with ab187144 at 1/100 and DAPI staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320-103] (ab187144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320-103] (ab187144)

    Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling Emi1 with ab187144 at 1/100 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.

  • Anti-Emi1 antibody [EPR15320-103] (ab187144)
    Anti-Emi1 antibody [EPR15320-103] (ab187144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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