Anti-Emi1 antibody (ab244426)
Key features and details
- Rabbit polyclonal to Emi1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Emi1 antibody
See all Emi1 primary antibodies -
Description
Rabbit polyclonal to Emi1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Emi1 aa 150-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UKT4 -
Positive control
- IHC-P: Human bone marrow, lymph node, and kidney tissues; WB: U-251 MG whole cell lysate; ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody (ab244426)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bone marrow tissue labelling Emi1 with ab244426 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-Emi1 antibody (ab244426)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Emi1 (green) using ab244426 at 4 µg/ml in ICC.
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Western blot - Anti-Emi1 antibody (ab244426)Anti-Emi1 antibody (ab244426) at 0.4 µg/ml + U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Predicted band size: 50 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody (ab244426)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling Emi1 with ab244426 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody (ab244426)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Emi1 with ab244426 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody (ab244426)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Emi1 with ab244426 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
