Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ELKS antibody [EPR13777] - BSA and Azide free (ab250209)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13777] to ELKS - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ELKS antibody [EPR13777] - BSA and Azide free
    See all ELKS primary antibodies

  • Description

    Rabbit monoclonal [EPR13777] to ELKS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250209 is the carrier-free version of ab180507. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250209 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ELKS antibody [EPR13777] - BSA and Azide free (ab250209)
    Western blot - Anti-ELKS antibody [EPR13777] - BSA and Azide free (ab250209)
    All lanes : Anti-ELKS antibody [EPR13777] (ab180507) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK293 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SW480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 128 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180507, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-ELKS antibody [EPR13777] - BSA and Azide free (ab250209)
    Immunocytochemistry - Anti-ELKS antibody [EPR13777] - BSA and Azide free (ab250209)
    This data was developed using ab180507, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling ELKS (red) with ab180507 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/250 dilution. Nuclei were counterstained with DAPI (blue).
  • Anti-ELKS antibody [EPR13777] - BSA and Azide free (ab250209)
    Anti-ELKS antibody [EPR13777] - BSA and Azide free (ab250209)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.