Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PARN antibody [EPR11670(2)] (ab188333)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11670(2)] to PARN
  • Suitable for: IP, ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PARN antibody [EPR11670(2)]
    See all PARN primary antibodies

  • Description

    Rabbit monoclonal [EPR11670(2)] to PARN

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, Hela, MDA-MB435, PC-12, NIH-3T3 cell lysates; IHC-P: Human papillary adenocarcinoma of thyroid, Mouse liver; ICC/IF: Hela cells; Flow Cyt: Hela cells; IP: K562 celll lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11670(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Western blot - Anti-PARN antibody [EPR11670(2)] (ab188333)
    All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MDA-MB-435 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] (ab188333)

    Immunofluorescent analysis of HeLa cells labelling PARN with ab188333 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue). 

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Western blot - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Western blot - Anti-PARN antibody [EPR11670(2)] (ab188333)
    All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] (ab188333)

    Immunohistochemical analysis of formalin fixed paraffin embedded Human papillary adenocarcinoma of thyroid labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] (ab188333)

    Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG.  Counterstained with Haematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] (ab188333)

    Immunohistochemical analysis of Hela cells fixed in 4% paraformaldehyde labeling PARN with ab188333 at 1/100 and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution.  Counterstained with DAPI (blue).

  • Flow Cytometry - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Flow Cytometry - Anti-PARN antibody [EPR11670(2)] (ab188333)

    Flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling PARN with ab188333 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution.  Rabbit monoclonal IgG was used as an isotype control.

  • Immunoprecipitation - Anti-PARN antibody [EPR11670(2)] (ab188333)
    Immunoprecipitation - Anti-PARN antibody [EPR11670(2)] (ab188333)

    Immunoprecipitation of K562 cell lysate labeling PARN with ab188333 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

  • Anti-PARN antibody [EPR11670(2)] (ab188333)
    Anti-PARN antibody [EPR11670(2)] (ab188333)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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