Anti-eIF3g antibody [EPR16146] - BSA and Azide free (ab251075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16146] to eIF3g - BSA and Azide free
- Suitable for: ICC, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-eIF3g antibody [EPR16146] - BSA and Azide free
See all eIF3g primary antibodies -
Description
Rabbit monoclonal [EPR16146] to eIF3g - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251075 is the carrier-free version of ab191422 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251075 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as EIF3S4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR16146 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using ab191422, the same antibody clone in a different buffer formulation.
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All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/1000 dilution
Lane 1 : PC-12 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using ab191422, the same antibody clone in a different buffer formulation.
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This data was developed using ab191422, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab191422, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded rat skeletal muscle tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab191422, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling eIF3g using ab191422 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100. The two negative controls: Primary ab concentration (anti-EIF3G), Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
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This data was developed using ab191422, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of 2% paraformaldehyde fixed 293T cells labeling eIF3g using ab191422 at a 1/80 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green).
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