Antibodies, Proteins, Kits and Reagents for Life Science

Anti-EGFR (phospho S1142) antibody (ab76194)

Key features and details

Rabbit polyclonal to EGFR (phospho S1142)
Suitable for: WB, ICC/IF
Reacts with: Human
Isotype: IgG

Product name

Anti-EGFR (phospho S1142) antibody
See all EGFR primary antibodies
Description

Rabbit polyclonal to EGFR (phospho S1142)
Host species

Rabbit
Specificity

ab76194 is not predicted to react with other ErbB family members.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human EGFR conjugated to Keyhole Limpet Haemocyanin (KLH). The aa numbering is around serine 1166 if the signal peptide is included.
Database link: P00533
Positive control
- IHC-P: A431 cells untreated or treated with Calyculin A (50 nM) for 15 minutes. WB: A431 cell lystes treated with Calyculin A (100 nM) for 30 minutes.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab76194 was cross adsorbed to unphosphorylated EGFR (serine 1142) coupled to agarose then affinity purified using phospho EGFR (serine 1142) peptide (without carrier).
Clonality

Polyclonal
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1142) antibody (ab76194)

ab76194, at a 1/200 dilution, staining EGFR in A431 cells by Immunoflurescence.
Left image: untreated.
Middle image: treated with Calyculin A (50 nM) for 15 minutes.
Right image: treated with Calyculin A (50 nM) for 15 minutes with blocking peptide, phospho EGFR (Ser-1142).
Western blot - Anti-EGFR (phospho S1142) antibody (ab76194)

All lanes : Anti-EGFR (phospho S1142) antibody (ab76194) at 1/1000 dilution

Lane 1 : A431 cells treated with
Calyculin A (100 nM) for 30 minutes
Lane 2 : A431 cells treated with
Calyculin A (100 nM) for 30 minutes and then treated with lambda phosphatase

Predicted band size: 134 kDa
Observed band size: 180 kDa
why is the actual band size different from the predicted?

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