Recombinant human EGFR protein (Fc Chimera) (ab84002)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human EGFR protein (Fc Chimera)
See all EGFR proteins and peptides -
Biological activity
The ED50 of ab84002 is typically 60-100 ng/ml as measured by its ability to neutralize EGF mediated proliferation of murine NIH3T3 fibroblasts. -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical sequence: LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLG NLEITYVQR NYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNM YYENSYALA VLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNV ESIQWRDIV SSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENC QKLTKIICA QQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFR DEATCKDTC PPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTD HGSCVRACG ADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSI NATNIKHFK NCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQ AWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITS LGLRSLKEI SDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRG ENSCKATGQ VCHALCSPEGCWGPEPRDCVSRSSNTKVDKKVEPKSCDK THTCPPCPA PELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHED PEVKFNWYV DGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKE YKCKVSNKA LPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLT CLVKGFYPS DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK -
Amino acids
1 to 525 -
Additional sequence information
Fused with the Fc region of Human IgG1 at the C-terminus.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Images
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SDS-PAGE - Recombinant human EGFR protein (Fc Chimera) (ab84002)Lane 1 – MW markers; Lane 2 – ab84002; Lane 3 – ab84002 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab84002 treated with a glycosidase cocktail to remove potential N and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Deep Purple™.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
Functional Studies - Recombinant human EGFR protein (Fc Chimera) (ab84002)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
The triangle indicates theoretical pI and MW of the protein.
