Anti-Malondialdehyde antibody [11E3] (ab243066)
![Anti-Malondialdehyde antibody [11E3] (ab243066)](https://www.abcam.com/ps/products/243/ab243066/Images/ab243066-451404-anti-malondialdehyde-antibody-11e3-immunohistochemistry-kidney-mouse.jpg "Anti-Malondialdehyde antibody [11E3] (ab243066)")
Key features and details
- Mouse monoclonal [11E3] to Malondialdehyde
- Suitable for: WB, IHC
- Isotype: IgG1
Overview
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Product name
Anti-Malondialdehyde antibody [11E3]
See all Malondialdehyde primary antibodies -
Description
Mouse monoclonal [11E3] to Malondialdehyde -
Host species
Mouse -
Tested applications
Suitable for: WB, IHCmore details -
Immunogen
Chemical/ Small Molecule corresponding to Malondialdehyde conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: Malondialdehyde-BSA; HeLa cells (+/- hydrogen peroxide treatment) cell lysate. IHC: Mouse kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
11E3 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry - Anti-Malondialdehyde antibody [11E3] (ab243066)
Immunohistochemical analysis of mouse kidney tissue labeling Malondialdehyde with ab243066 (green). Tissue was incubated with ab243066 at 1/100 overnight at 4oC then for 30 minutes at 37oC, followed by incubation with a Goat anti-Mouse IgG (H+L):FITC secondary antibody for 45 minutes at 37oC. Counterstain used was DAPI for 3 minutes at RT. Magnification: 5X.
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![Western blot - Anti-Malondialdehyde antibody [11E3] (ab243066)](https://www.abcam.com/ps/products/243/ab243066/Images/ab243066-332387-anti-malondialdehyde-antibody-11e3-western-blot.png)
Western blot - Anti-Malondialdehyde antibody [11E3] (ab243066)All lanes : Anti-Malondialdehyde antibody [11E3] (ab243066) at 1/1000 dilution
Lane 1 : Malondialdehyde-BSA at 0.5 µg
Lane 2 : Malondialdehyde-BSA at 2 µg
Lane 3 : BSA at 0.5 µg
Lane 4 : BSA at 2 µg
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hrs at RT.
Secondary incubation for 1 hr at RT.
Block: 5% Skimmed milk in TBST.
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![Western blot - Anti-Malondialdehyde antibody [11E3] (ab243066)](https://www.abcam.com/ps/products/243/ab243066/Images/ab243066-332388-anti-malondialdehyde-antibody-11e3-western-blot.png)
Western blot - Anti-Malondialdehyde antibody [11E3] (ab243066)All lanes : Anti-Malondialdehyde antibody [11E3] (ab243066) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (treated with hydrogen peroxide) cell lysate
Lysates/proteins at 12 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.