Anti-EGFR (mutated L858R) antibody [EPR15348] - BSA and Azide free (ab251130)
![Anti-EGFR (mutated L858R) antibody [EPR15348] - BSA and Azide free (ab251130)](https://www.abcam.com/ps/products/251/ab251130/Images/ab251130-407798-anti-egfr-mutated-l585r-antibody-epr15348-bsa-and-azide-free-western-blot.jpg "Anti-EGFR (mutated L858R) antibody [EPR15348] - BSA and Azide free (ab251130)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15348] to EGFR (mutated L858R) - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-EGFR (mutated L858R) antibody [EPR15348] - BSA and Azide free
See all EGFR primary antibodies -
Description
Rabbit monoclonal [EPR15348] to EGFR (mutated L858R) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251130 is the carrier-free version of ab192263. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251130 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15348 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (mutated L585R) antibody [EPR15348] - BSA and Azide free (ab251130)All lanes : Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263) at 1/1000 dilution
Lane 1 : H1975 cell lysate
Lane 2 : NCI-H460 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 134 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?This data was developed using ab192263, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-EGFR (mutated L585R) antibody [EPR15348] - BSA and Azide free (ab251130)](https://www.abcam.com/ps/products/251/ab251130/Images/ab251130-2-anti-egfr-mutated-l585r-antibody-epr15348-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-EGFR (mutated L585R) antibody [EPR15348] - BSA and Azide free (ab251130)This data was developed using ab192263, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling EGFR with ab192263 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue). The negative controls are as follows;
1. ab192263 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor® 594) at 1/500 dilution.
2. ab192263 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor® 594) at 1/500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (mutated L585R) antibody [EPR15348] - BSA and Azide free (ab251130)](https://www.abcam.com/ps/products/251/ab251130/Images/ab251130-1-anti-egfr-mutated-l585r-antibody-epr15348-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (mutated L585R) antibody [EPR15348] - BSA and Azide free (ab251130)This data was developed using ab192263, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling EGFR with ab192263 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control also shown. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Anti-EGFR (mutated L858R) antibody [EPR15348] - BSA and Azide free (ab251130)](https://www.abcam.com/ps/products/251/ab251130/Images/ab251130-4-benefits-of-recombinant-antibodies.png)
Anti-EGFR (mutated L858R) antibody [EPR15348] - BSA and Azide free (ab251130)