Anti-ECHS1 antibody [EPR11785] (ab174312)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11785] to ECHS1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-ECHS1 antibody [EPR11785]
See all ECHS1 primary antibodies -
Description
Rabbit monoclonal [EPR11785] to ECHS1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ECHS1 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P30084 -
Positive control
- MCF7, HeLa, 293T, HepG2, Human fetal liver and fetal muscle lysates; Human heart tissue; Human liver tissue; MCF7 cell
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11785 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ECHS1 antibody [EPR11785] (ab174312) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Human fetal liver lysate
Lane 6 : Human fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa
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Immunofluorescent staining of MCF7 cells labeling ECHS1 with ab174312 at 1/250 dilution (red). DAPI nuclear staining (blue).
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Immunohistochemical analysis of paraffin embedded Human liver tissue labaling ECHS1 with ab174312 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ECHS1 with ab174312 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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