Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ECHS1 antibody [EPR11785] (ab174312)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11785] to ECHS1
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Human

Product name

Anti-ECHS1 antibody [EPR11785]
See all ECHS1 primary antibodies
Description

Rabbit monoclonal [EPR11785] to ECHS1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ECHS1 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P30084
Positive control
- MCF7, HeLa, 293T, HepG2, Human fetal liver and fetal muscle lysates; Human heart tissue; Human liver tissue; MCF7 cell
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11785
Isotype

IgG
Research areas

Western blot - Anti-ECHS1 antibody [EPR11785] (ab174312)

All lanes : Anti-ECHS1 antibody [EPR11785] (ab174312) at 1/10000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Human fetal liver lysate
Lane 6 : Human fetal muscle lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 31 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ECHS1 antibody [EPR11785] (ab174312)

Immunofluorescent staining of MCF7 cells labeling ECHS1 with ab174312 at 1/250 dilution (red). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11785] (ab174312)

Immunohistochemical analysis of paraffin embedded Human liver tissue labaling ECHS1 with ab174312 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11785] (ab174312)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ECHS1 with ab174312 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-ECHS1 antibody [EPR11785] (ab174312)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com