Anti-ECHS1 antibody (ab228631)
Key features and details
- Rabbit polyclonal to ECHS1
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ECHS1 antibody
See all ECHS1 primary antibodies -
Description
Rabbit polyclonal to ECHS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human ECHS1 (internal sequence). The exact sequence is proprietary.
Database link: P30084 -
Positive control
- WB: Mouse brain tissue whole cell lysate; HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Mouse heart, ovary and liver tissue. ICC/IF: A431 and HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ECHS1 antibody (ab228631)
A431 (human epidermoid carcinoma cell line) cells stained for ECHS1 (green) using ab228631 at 1/200 dilution in ICC/IF. Cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 minutes.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody (ab228631)Paraffin-embedded mouse liver tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-ECHS1 antibody (ab228631)Anti-ECHS1 antibody (ab228631) at 1/1000 dilution + Mouse brain tissue whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa12% SDS-PAGE
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Western blot - Anti-ECHS1 antibody (ab228631)All lanes : Anti-ECHS1 antibody (ab228631) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa12% SDS-PAGE
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Immunoprecipitation - Anti-ECHS1 antibody (ab228631)ECHS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg ab228631. Western blot was performed from the immunoprecipitate using ab228631.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab228631 in HEK-293T whole cell extract.
Lane 3: ab228631 IP in HEK-293T whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody (ab228631)Paraffin-embedded mouse heart tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody (ab228631)Paraffin-embedded mouse ovary tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody (ab228631)Paraffin-embedded mouse liver tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ECHS1 antibody (ab228631)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ECHS1 (green) using ab228631 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
