The MarkerGene™LysoLive™ Lysosomal Metabolic Health Assay Kit utilizes the sensitive substrate EsterGreen™ to stain only actively metabolizing lysosomes.

Lysosomes are acidic cytoplasmic organelles that are present in all nucleated mammalian cells. Lysosomes have been found to be involved in a variety of cellular processes including repair of the plasma membrane, defense against pathogens, cholesterol homeostasis, bone remodeling, metabolism, apoptosis, and cell signaling. Defects in lysosomal enzyme activity have been associated with a variety of diseases including Parkinson’s, Tay-Sachs, Sandhoff, Krabbe, Wolman, and Gaucher syndromes. Marker Gene has developed lysosomal staining compounds that are useful for labeling lysosomes in a live-cell format and are capable of monitoring lysosomal metabolic activity. These new targeted substrates are based upon fluorescent probes that have a low pKa value for optimal fluorescence at the lower physiological pH values found in the lysosomes as well as targeting groups to direct their accumulation to the lysosomes using a live-cell staining format. 

This kit is sold by Marker Gene under product code M1910.