Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
![Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-446358-anti-dynamin-1-antibody-ep801y-bsa-and-azide-free-western-blot.jpg "Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP801Y] to Dynamin 1 - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free
See all Dynamin 1 primary antibodies -
Description
Rabbit monoclonal [EP801Y] to Dynamin 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247322 is the carrier-free version of ab52611. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247322 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP801Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (purified) + C6 cell lysate at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDaThis data was developed using ab52611, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-445538-anti-dynamin-1-antibody-ep801y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDaThis data was developed using ab52611, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-5-anti-dynamin-1-antibody-ep801y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunofluorescence staining of Neuro-2a cells with purified ab52611 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52611 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-4-anti-dynamin-1-antibody-ep801y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-3-anti-dynamin-1-antibody-ep801y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-402257-anti-dynamin-1-antibody-ep801y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (unpurified) + 3T3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDaThis data was developed using ab52611, the same antibody clone in a different buffer formulation.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-1-anti-dynamin-1-antibody-ep801y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322) -
![Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)](https://www.abcam.com/ps/products/247/ab247322/Images/ab247322-6-benefits-of-recombinant-antibodies.png)
Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
