Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP801Y] to Dynamin 1 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free
    See all Dynamin 1 primary antibodies

  • Description

    Rabbit monoclonal [EP801Y] to Dynamin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247322 is the carrier-free version of ab52611. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247322 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (purified) + C6 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    This data was developed using ab52611, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    This data was developed using ab52611, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Immunocytochemistry - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunofluorescence staining of Neuro-2a cells with purified ab52611 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52611 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (unpurified) + 3T3 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    This data was developed using ab52611, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    This data was developed using ab52611, the same antibody clone in a different buffer formulation.Unpurified ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.
  • Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)
    Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

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