Anti-Synip antibody [EP302Y] (ab40674)
![Anti-Synip antibody [EP302Y] (ab40674)](https://www.abcam.com/ps/products/40/ab40674/Images/ab40674-53623-anti-synip-antibody-ep302y-immunohistochemistry.jpg "Anti-Synip antibody [EP302Y] (ab40674)")
Key features and details
- Rabbit monoclonal [EP302Y] to Synip
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Synip antibody [EP302Y] -
Description
Rabbit monoclonal [EP302Y] to Synip -
Host species
Rabbit -
Specificity
This antibody is specific for Synip. It is predicted to detect the isoform 2 based an sequence analysis. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Synip (C terminal). The exact sequence is proprietary.
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Positive control
- Human skeletal lysate and human lung carcinoma tissue.
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General notes
Previously labelled as Syntaxin-binding protein 4.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP302Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
ab40674 at a 1:250 dilution staining Synip in human lung carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Synip antibody [EP302Y] (ab40674)](https://www.abcam.com/ps/products/40/ab40674/Images/ab40674-53624-anti-synip-antibody-ep302y-western-blot.jpg)
Western blot - Anti-Synip antibody [EP302Y] (ab40674)Anti-Synip antibody [EP302Y] (ab40674) at 1/5000 dilution + human skeletal lysate
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?