Anti-Dnmt1 antibody [2C1] - BSA and Azide free (ab255781)
![Anti-Dnmt1 antibody [2C1] - BSA and Azide free (ab255781)](https://www.abcam.com/ps/products/255/ab255781/Images/ab255781-383114-AntiDnmt1-antibody-2C1-wb-various-human-cells.jpg "Anti-Dnmt1 antibody [2C1] - BSA and Azide free (ab255781)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [2C1] to Dnmt1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Dnmt1 antibody [2C1] - BSA and Azide free
See all Dnmt1 primary antibodies -
Description
Rat monoclonal [2C1] to Dnmt1 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment (His-tag) within Human Dnmt1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P26358 -
Positive control
- WB: NCI-H1299, HEK-293, HeLa, Jurkat and HepG2 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255781 is the carrier-free version of ab252825. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2C1 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Dnmt1 antibody [2C1] - BSA and Azide free (ab255781)All lanes : Anti-Dnmt1 antibody [2C1] (ab252825) at 0.353 µg/ml
Lane 1 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 5 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution
Predicted band size: 183 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
This image was produced using the same antibody clone but in a different formulation ab252825, PBS, sodium azide, glycerol and BSA.
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![Anti-Dnmt1 antibody [2C1] - BSA and Azide free (ab255781)](https://www.abcam.com/ps/products/255/ab255781/Images/ab255781-2-benefits-of-recombinant-antibodies.png)
Anti-Dnmt1 antibody [2C1] - BSA and Azide free (ab255781)