Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DMAP1 antibody [EPR18179] (ab188407)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18179] to DMAP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DMAP1 antibody [EPR18179]
    See all DMAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18179] to DMAP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, 293, MCF7, Ramos, Neuro-2a, Raw264.7, PC12, NIH 3T3 cell lysates; HeLa nuclear fraction lysate. ICC/IF: HeLa and F9 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)
    Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)
    Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic carcinoma cell line) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on F9 cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

  • Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
    Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
    All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 5 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
    Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
    All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilution

    Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
    Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
    All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-DMAP1 antibody [EPR18179] (ab188407)
    Anti-DMAP1 antibody [EPR18179] (ab188407)

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