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Recombinant Human MGMT protein (ab185395)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human MGMT protein
    See all MGMT proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Purity is determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P16455

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMDKDCEMKRTTLDSPLGKLELSGCEQGLHE IKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFP VPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAV GGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPG LGGSSGLAGAWLKGAGATSGSPPAGRN

    • Predicted molecular weight

      24 kDa including tags

    • Amino acids

      1 to 207

    • Tags

      His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab185395 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris-HCl buffer, 0.03% EDTA

    General Info

    • Alternative names

      • 6 O methylguanine DNA methyltransferase
      • 6-O-methylguanine-DNA methyltransferase
      • Agat
      • AGT
      • AI267024
      • EC 2.1.1.63
      • Methylated DNA protein cysteine methyltransferase
      • Methylated-DNA--protein-cysteine methyltransferase
      • Methylguanine DNA methyltransferase
      • MGC107020
      • MGMT
      • MGMT_HUMAN
      • O 6 methylguanine DNA alkyltransferase
      • O 6 methylguanine DNA methyltransferase
      • O-6-methylguanine-DNA methyltransferase
      • O-6-methylguanine-DNA-alkyltransferase
      see all

    • Function

      Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

    • Sequence similarities

      Belongs to the MGMT family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab185395? Please let us know so that we can cite the reference in this datasheet.

ab185395 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris-HCl buffer, 0.03% EDTA

Images

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