Recombinant human DNMT3B + DNMT3L protein (ab198133)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human DNMT3B + DNMT3L protein -
Biological activity
Assayed using DNMT3B Assay Kit. Add 50 µl reaction mix containing DNMT3B/DNMT3L to wells coated with DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.
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Purity
> 85 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
WRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFL ESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGHTCDRPPSWY LFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTI PDVHGGSLQNAVRVWSNIPAIRSSRHWALVSEEELSLLAQNKQSSKLAAK WPTKLVKNCFLPLREYFKYFSTELTSSL -
Predicted molecular weight
27 kDa -
Amino acids
160 to 387 -
Tags
His tag N-Terminus -
Additional sequence information
NM_013369.
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Amino Acid Sequence 2
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Species
Human -
Sequence
YPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAV GTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKG LYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRF LECNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYN RIAKLKKVQTITTKSNSIKQGKNQLFPVVMNGKEDVLWCTELERIFGFPV HYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE -
Predicted molecular weight
59 kDa including tags -
Amino acids
564 to 853 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_006892.
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Preparation and Storage
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Images
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Functional Studies - Recombinant human DNMT3B + DNMT3L protein (ab198133)
Functional assay of ab198133.
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SDS-PAGE - Recombinant human DNMT3B + DNMT3L protein (ab198133)10% SDS-PAGE analysis of ab198133 with Coomassie staining.
Lane 1: ab198133
Lane 2: Protein marker