Anti-Dnmt1 antibody (ab87654)
Key features and details
- Rabbit polyclonal to Dnmt1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Dnmt1 antibody
See all Dnmt1 primary antibodies -
Description
Rabbit polyclonal to Dnmt1 -
Host species
Rabbit -
Specificity
ab87656 is recommended for DNMT1 detection in WB. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Highly purified recombinant Mouse Dnmt1 (amino acids 1-248), soluble form
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General notes
Useful for analysis of the protein complex containing Dnmt1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Traces of Ammonium sulphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Useful for analysis of the protein complex containing Dnmt1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
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All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Tagged-NP 95 + BirA Input
Lane 2 : Tagged-NP 95 + BirA Beads
Lane 3 : BirA Input
Lane 4 : BirA Beads
Predicted band size: 183 kDa
Observed band size: 183 kDa
Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna. -
All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Dnmt1 1+/+ (J1) (nuclear extract)
Lane 2 : Dnmt1 1-/- (36-2D6) (nuclear extract)
Lane 3 : Tagged-NP95 + BirA (0.1M KCl fraction)
Lane 4 : BirA (0.1M KCl fraction)
Predicted band size: 183 kDa
Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.