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Epigenetics and Nuclear Signaling DNA methylation DNMTs

Anti-Dnmt1 antibody (ab87654)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Dnmt1 antibody (ab87654)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Dnmt1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Dnmt1 antibody
    See all Dnmt1 primary antibodies
  • Description

    Rabbit polyclonal to Dnmt1
  • Host species

    Rabbit
  • Specificity

    ab87656 is recommended for DNMT1 detection in WB.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Highly purified recombinant Mouse Dnmt1 (amino acids 1-248), soluble form

  • General notes


    Useful for analysis of the protein complex containing Dnmt1.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6
    Preservative: 0.05% Sodium azide
    Constituent: 50% Glycerol

    Traces of Ammonium sulphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Useful for analysis of the protein complex containing Dnmt1.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody (ab87654)
    Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody (ab87654)
    ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
  • Western blot - Anti-Dnmt1 antibody (ab87654)
    Western blot - Anti-Dnmt1 antibody (ab87654)
    All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml

    Lane 1 : Tagged-NP 95 + BirA Input
    Lane 2 : Tagged-NP 95 + BirA Beads
    Lane 3 : BirA Input
    Lane 4 : BirA Beads

    Predicted band size: 183 kDa
    Observed band size: 183 kDa



    Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna.
  • Western blot - Anti-Dnmt1 antibody (ab87654)
    Western blot - Anti-Dnmt1 antibody (ab87654)
    All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml

    Lane 1 : Dnmt1 1+/+ (J1) (nuclear extract)
    Lane 2 : Dnmt1 1-/- (36-2D6) (nuclear extract)
    Lane 3 : Tagged-NP95 + BirA (0.1M KCl fraction)
    Lane 4 : BirA (0.1M KCl fraction)

    Predicted band size: 183 kDa



    Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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