DNMT Activity Assay Kit (Fluorometric) (ab113468)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr 45 min
Overview
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Product name
DNMT Activity Assay Kit (Fluorometric)
See all DNMT kits -
Detection method
Fluorescent -
Assay type
Enzyme activity -
Assay time
3h 45m -
Species reactivity
Reacts with: Mouse, Human, Plants
Predicted to work with: Mammals, Fungi
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Product overview
DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment. -
Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Adomet, 50X 1 x 60µl 1 x 120µl Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl DNMT Assay Buffer 1 x 4ml 1 x 8ml DNMT Enzyme Control, 50 µg/mL 1 x 6µl 1 x 12µl Enhancer Solution 1 x 6µl 1 x 12µl Fluoro Developer 1 x 6µl 1 x 12µl Fluoro Diluter 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 6µl 1 x 12µl -
Research areas
Images
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Functional Studies - DNMT Activity Quantification Kit (Fluorometric) (ab113468)Demonstration of high sensitivity and specificity achieved by using recombinant DNMT1 with ab113468.
