Anti-Rheumatoid Factor IgM ELISA Kit (ab178653)
Key features and details
- Sensitivity: 0.5 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
Overview
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Product name
Anti-Rheumatoid Factor IgM ELISA Kit -
Detection method
Colorimetric -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
Assay duration
Multiple steps standard assayProduct overview
Abcam’s anti-Rheumatoid Factor IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to Rheumatoid Factor IgM in Human serum or plasma.
The quantitative immunoenzymatic determination of IgM rheumatoid factors is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strips are precoated with Fc fragments of Human immunoglobulin G to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added. This conjugate binds to the captured rheumatoid factors. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of IgM rheumatoid factors in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Platform
MicroplateProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Fc Fragment Coated Microplate (12 x 8 wells) 1 unit IgG Sample Diluent 1 x 100ml Rheumatoid Factor Anti-IgM HRP Conjugate 1 x 20ml Rheumatoid Factor IgM Negative Control 1 x 2ml Rheumatoid Factor IgM Positive Control 1 x 2ml Rheumatoid Factor IgM Standard A - 0 U/mL 1 x 2ml Rheumatoid Factor IgM Standard B - 3 U/mL 1 x 2ml Rheumatoid Factor IgM Standard C - 10 U/mL 1 x 2ml Rheumatoid Factor IgM Standard D - 30 U/mL 1 x 2ml Rheumatoid Factor IgM Standard E - 100 U/mL 1 x 2ml Rheumatoid Factor IgM Standard F - 300 U/mL 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Fc Fragment Coated Microplate (12 x 8 wells) 1 unit IgG Sample Diluent 1 x 100ml Rheumatoid Factor Anti-IgM HRP Conjugate 1 x 20ml Rheumatoid Factor IgM Negative Control 1 x 2ml Rheumatoid Factor IgM Positive Control 1 x 2ml Rheumatoid Factor IgM Standard A - 0 U/mL 1 x 2ml Rheumatoid Factor IgM Standard B - 3 U/mL 1 x 2ml Rheumatoid Factor IgM Standard C - 10 U/mL 1 x 2ml Rheumatoid Factor IgM Standard D - 30 U/mL 1 x 2ml Rheumatoid Factor IgM Standard E - 100 U/mL 1 x 2ml Rheumatoid Factor IgM Standard F - 300 U/mL 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative Standard Curve using ab178653.