Anti-DNA Gyrase A antibody (ab75594)
Key features and details
- Rabbit polyclonal to DNA Gyrase A
- Suitable for: WB
- Reacts with: Escherichia coli, Staphylococcus aureus
- Isotype: IgG
Overview
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Product name
Anti-DNA Gyrase A antibody -
Description
Rabbit polyclonal to DNA Gyrase A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli, Staphylococcus aureus -
Immunogen
Purified E. coli DNA Gyrase A protein (over expressed from E. coli)
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Positive control
- E.coli cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
The antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNA Gyrase A antibody (ab75594)All lanes : Anti-DNA Gyrase A antibody (ab75594) at 1/1000 dilution
Lane 1 : E coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
Lane 2 : E coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
Lane 3 : E coli cell lysate (Gyrase B, whole protein) at 1 µg
Lane 4 : E coli cell lysate (Gyrase B, whole protein) at 0.5 µg
Lane 5 : E coli cell lysate (Gyrase B, whole protein) at 0.1 µg
Lane 6 : E coli cell lysate (Gyrase A, whole protein) at 1 µg
Lane 7 : E coli cell lysate (Gyrase A, whole protein) at 0.5 µg
Lane 8 : E coli cell lysate (Gyrase A, whole protein) at 0.1 µg
Secondary
All lanes : goat anti rabbit Igs at 1/5000 dilution
Observed band size: 97 kDa why is the actual band size different from the predicted?