Anti-DLL1 antibody (ab10554)
Key features and details
- Rabbit polyclonal to DLL1
- Suitable for: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DLL1 antibody
See all DLL1 primary antibodies -
Description
Rabbit polyclonal to DLL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide:
CGEEWSQDLHSSGRTDLRYS
conjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. The product is sterile, filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554) Greene M et al., PLos One, 2016 Sep 13;11(9):e0161157. Fig 1.; doi: 10.1371/journal.pone.0161157 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
ab10554 staining DLL1 in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 4% BSA for 1 hour. Samples were incubated with primary antibody for 1 hour at room temperature. DAPI used to stain nuclei
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL1 antibody (ab10554)ab10554 staining DLL1 in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (2ug/ml) for 1.5 hours at room temperature. A peroxidase-conjugated polyclonal was used as the secondary antibody. Nuclei stained purple with hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)ICC/IF image of ab10554 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10554, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-DLL1 antibody (ab10554)Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
Lane 2: Mouse Pancreas
Lane 3: Mouse Brain
Lane 4: Rat Heart
Lane 5: Rat Pancreas
Lane 6: Rat BrainSecondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 1 minute
Expected molecular weight: 77kDa
Cell lysates at 20µ g per lane.NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.
Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
