Anti-Transferrin antibody [EPR18240-29] (ab278498)
![Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449859-AntiTransferrin-antibodyWB1.jpg "Anti-Transferrin antibody [EPR18240-29] (ab278498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18240-29] to Transferrin
- Suitable for: WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse
Overview
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Product name
Anti-Transferrin antibody [EPR18240-29]
See all Transferrin primary antibodies -
Description
Rabbit monoclonal [EPR18240-29] to Transferrin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt (Intra) MouseICC MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse liver, kidney, testis tissue lysate and plasma; Hepa1-6 whole cell lysate ICC: Hepa1-6 cells. Flow Cyt: Hepa1-6 (Mouse hepatoma epithelial cells)
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General notes
Please note: Clone [EPR18240-29] (this product) is different to that of ab277635 [EPR20149-224] for the same target.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18240-29 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)All lanes : Anti-Transferrin antibody [EPR18240-29] (ab278498) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 77 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1-3: 3.25 seconds; Lane 4: 70 seconds
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![Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449860-AntiTransferrin-antibodyWB2.jpg)
Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)Anti-Transferrin antibody [EPR18240-29] (ab278498) at 1/1000 dilution + Mouse plasma at 20 µl
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds.
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![Immunocytochemistry - Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449861-AntiTransferrin-antibodyICC1.jpg)
Immunocytochemistry - Anti-Transferrin antibody [EPR18240-29] (ab278498)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 cells labelling Transferrin with ab278498 at 1/100 (5.52 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Hepa1-6 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449862-AntiTransferrin-antibodyFC1.jpg)
Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR18240-29] (ab278498)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (Mouse hepatoma epithelial cell) cells labelling Transferrin with ab278498 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.