Anti-DIS3 antibody - C-terminal (ab226204)
Key features and details
- Rabbit polyclonal to DIS3 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIS3 antibody - C-terminal
See all DIS3 primary antibodies -
Description
Rabbit polyclonal to DIS3 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human DIS3 aa 858-908 (C terminal). The exact sequence is proprietary. NP_055768.3
Database link: Q9Y2L1 -
Positive control
- IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysate.
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General notes
This product was previously labelled as DIS3, Dis3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DIS3 antibody - C-terminal (ab226204)All lanes : Anti-DIS3 antibody - C-terminal (ab226204) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-DIS3 antibody - C-terminal (ab226204)DIS3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226204 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226204 at 1 µg/ml.
Lane 1: ab226204 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
