Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free
    See all Dihydrofolate reductase (DHFR) primary antibodies

  • Description

    Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248545 is the carrier-free version of ab133546. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248545 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
    Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
    Lane 1 : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution (purified)
    Lanes 2-5 : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using ab133546, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
    Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
    All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa



    This data was developed using ab133546, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
    OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
    This data was developed using ab133546, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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