Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17628] to Delta-catenin - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free
    See all Delta-catenin primary antibodies

  • Description

    Rabbit monoclonal [EPR17628] to Delta-catenin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250781 is the carrier-free version of ab184917 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250781 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as delta 2 Catenin

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    Immunocytochemistry - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    This data was developed using ab184917, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling delta-catenin with ab184917 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and nuclear staining on Neuro-2a cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab184917 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
  • Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    This data was developed using ab184917, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

    Lane 1 : Rat hippocampus lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    This data was developed using ab184917, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

     

    Exposure time: 3 minutes.

  • Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Mouse cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    This data was developed using ab184917, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)
    Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (ab250781)

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