Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22556-138] to Amylin/DAP - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free
    See all Amylin/DAP primary antibodies

  • Description

    Rabbit monoclonal [EPR22556-138] to Amylin/DAP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-Fr
    Rat
    IHC-P
    Human
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human, mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue. ICC/IF: Beta-TC-6 cells. Flow Cyt: Beta-TC-6 cells. IP: Beta-TC-6 whole cell lysate.

  • General notes

    Ab255329 is the carrier-free version of ab254259. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255329 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Amylin/DAP with ab254259 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the islet in human pancreas (PMID: 30014749). The section was incubated with ab254259 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Amylin/DAP with ab254259 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the islet in mouse pancreas (PMID: 30014749). The section was incubated with ab254259 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Amylin/DAP with ab254259 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the islet in rat pancreas (PMID: 30014749). The section was incubated with ab254259 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).

  • Immunohistochemistry (Frozen sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Immunohistochemistry (Frozen sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling Amylin/DAP with ab254259 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on beta cells of mouse pancreatic islets (PMID: 14656003) is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).
  • Immunohistochemistry (Frozen sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Immunohistochemistry (Frozen sections) - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat pancreas tissue labeling Amylin/DAP with ab254259 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on beta cells of rat pancreatic islets (PMID: 14656003) is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).
  • Immunocytochemistry/ Immunofluorescence - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Immunocytochemistry/ Immunofluorescence - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labeling Amylin/DAP with ab254259 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Beta-TC-6 cell line. Negative control: NIH/3T3 (PMID:15802374). The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).
  • Flow Cytometry - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Flow Cytometry - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / Beta-TC-6 (Mouse pancreas insulinoma beta cell, Right) cell lines labeling Amylin/DAP with ab254259 at 1/500 (red) compared with a Rabbit monoclonal IgG isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Negative control: NIH/3T3 (PMID:15802374).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).

  • Immunoprecipitation - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Immunoprecipitation - Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

    Amylin/DAP was immunoprecipitated from 0.35 mg Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate with ab254259 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254259 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: Beta-TC-6 whole cell lysate 10 µg (Input).
    Lane 2: ab254259 IP in Beta-TC-6 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254259 in Beta-TC-6 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254259).

  • Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)
    Anti-Amylin/DAP antibody [EPR22556-138] - BSA and Azide free (ab255329)

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For licensing inquiries, please contact partnerships@abcam.com

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