Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR354(3)] to p53 (acetyl K305) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR354(3)] to p53 (acetyl K305) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects p53 acetylated on Lysine 305.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and T47-D cell lysates treated with etoposide and TSA, mouse kidney, mouse spleen and rat kidney tissue lysates.

  • General notes

    Ab226063 is the carrier-free version of ab109396. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226063 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)
    Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)
    All lanes : Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396) at 1/5000 dilution (purified)

    Lane 1 : Untreated T47-D cell lysate
    Lane 2 : T47-D cell lysate treated with Etoposide and TSA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109396, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)
    Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)
    All lanes : Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396) at 1/1000 dilution (purified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    This data was developed using ab109396, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)
    Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)
    All lanes : Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate, untreated
    Lane 2 : HepG2 cell lysate, treated with etoposide and TSA

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa



    This data was developed using ab109396, the same antibody clone in a different buffer formulation.

     

  • Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)
    Anti-p53 (acetyl K305) antibody [EPR354(3)] - BSA and Azide free (ab226063)

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