Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4 antibody [EPR24149-36] (ab259936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit Monoclonal [EPR24149-36] to Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4 antibody [EPR24149-36]

  • Description

    Rabbit Monoclonal [EPR24149-36] to Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebrum and astrocytoma tissue; Mouse cerebrum tissue; Rat cerebrum tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is  ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human astrocytoma. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum (PMID: 7736595). The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on rat liver (PMID: 7736595). The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    Western blot - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
    All lanes : Anti-Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4 antibody [EPR24149-36] (ab259936) at 1/1000 dilution

    Lane 1 : His-tagged mouse neuroligin 1 recombinant protein, 10 ng
    Lane 2 : LIF/His-tagged mouse neuroligin 2 recombinant protein, 10 ng
    Lane 3 : His-tagged mouse neuroligin 3 recombinant protein, 10 ng
    Lane 4 : His-tagged human neuroligin 4 recombinant protein, 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The rec proteins of neuroligin 1, neuroligin 2 and neuroligin 3 were made in house.

    The recombinant proteins were expressed from a mammalian - HEK-293 expression system.

    Exposure time: 3 minutes.

     

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