Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15636] to ATP5L2 + ATP5L - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free
    See all ATP5L2 + ATP5L primary antibodies

  • Description

    Rabbit monoclonal [EPR15636] to ATP5L2 + ATP5L - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment within Human ATP5L2 + ATP5L aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q7Z4Y8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    ab251071 is the carrier-free version of ab191417.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    Immunocytochemistry - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    This data was developed using ab191417, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).Negative controls: anti-ATP5L2 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.
  • Western blot - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    Western blot - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    All lanes : Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human pancreas tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa



    This data was developed using ab191417, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    This data was developed using ab191417, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    Immunoprecipitation - Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    This data was developed using ab191417, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab191417 at 1/100 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)
    Anti-ATP5L2 + ATP5L antibody [EPR15636] - BSA and Azide free (ab251071)

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