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Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)

Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18869] to GABARAPL1+GABARAPL3 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free
    See all GABARAPL1+GABARAPL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR18869] to GABARAPL1+GABARAPL3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251099 is the carrier-free version of ab191887. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251099 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR18869
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Opioid Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • GABA Receptors
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Images

  • Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution + Recombinant human GABARAPL1 full length protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab191887, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    In house recombinant Human GABARAPL1 is a GST-tagged full length protein (aa1-117).

  • Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution

    Lane 1 : Recombinant Human GABARAPL2 full length protein
    Lane 2 : Recombinant Human GABARAPL3 full length protein
    Lane 3 : Recombinant Human GABARAP (O95166) full length protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab191887, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All three recombinant Human full length proteins contain aa1-117 with an N-terminal His-Tag® and were made in-house.

  • Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    This data was developed using ab191887, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lanes 2-4: 30 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).

  • Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    This data was developed using ab191887, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).

  • Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab191887, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).

  • Immunoprecipitation - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Immunoprecipitation - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)

    This data was developed using ab191887, the same antibody clone in a different buffer formulation.

    GABARAPL1 + GABARAPL3 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab191887 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab191887 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input).

    Lane 2: ab191887 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191887 in HeLa whole cell lysate.

     Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     Exposure time: 30 seconds.

  • Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
    Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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