Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (ab251099)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18869] to GABARAPL1+GABARAPL3 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free
See all GABARAPL1+GABARAPL3 primary antibodies -
Description
Rabbit monoclonal [EPR18869] to GABARAPL1+GABARAPL3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251099 is the carrier-free version of ab191887. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251099 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR18869 -
Isotype
IgG -
Research areas
Images
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Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution + Recombinant human GABARAPL1 full length protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab191887, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
In house recombinant Human GABARAPL1 is a GST-tagged full length protein (aa1-117).
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution
Lane 1 : Recombinant Human GABARAPL2 full length protein
Lane 2 : Recombinant Human GABARAPL3 full length protein
Lane 3 : Recombinant Human GABARAP (O95166) full length protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab191887, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All three recombinant Human full length proteins contain aa1-117 with an N-terminal His-Tag® and were made in-house.
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?This data was developed using ab191887, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds; Lanes 2-4: 30 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?This data was developed using ab191887, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lane 2: 15 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab191887, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
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This data was developed using ab191887, the same antibody clone in a different buffer formulation.
GABARAPL1 + GABARAPL3 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab191887 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab191887 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10ug (Input).
Lane 2: ab191887 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191887 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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