Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-DDX41 antibody [EPR14298] (ab182007)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14298] to DDX41
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Human

Product name

Anti-DDX41 antibody [EPR14298]
See all DDX41 primary antibodies
Description

Rabbit monoclonal [EPR14298] to DDX41
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, Jurkat, A549 or Jar lysate, Human colon tissue or HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14298
Isotype

IgG
Research areas

Western blot - Anti-DDX41 antibody [EPR14298] (ab182007)

Anti-DDX41 antibody [EPR14298] (ab182007) at 1/10000 dilution + Jar cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DDX41 antibody [EPR14298] (ab182007)

Immunofluorescence analysis of HeLa cells (fixative -20℃ Acetone) labeling DDX41 with ab182007 at a 1/250 dilution. Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX41 antibody [EPR14298] (ab182007)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX41 with ab182007 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-DDX41 antibody [EPR14298] (ab182007)

All lanes : Anti-DDX41 antibody [EPR14298] (ab182007) at 1/2000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 70 kDa
Anti-DDX41 antibody [EPR14298] (ab182007)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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