Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDX41 antibody [EPR14298] (ab182007)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14298] to DDX41
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-DDX41 antibody [EPR14298]
    See all DDX41 primary antibodies

  • Description

    Rabbit monoclonal [EPR14298] to DDX41

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, A549 or Jar lysate, Human colon tissue or HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DDX41 antibody [EPR14298] (ab182007)
    Western blot - Anti-DDX41 antibody [EPR14298] (ab182007)
    Anti-DDX41 antibody [EPR14298] (ab182007) at 1/10000 dilution + Jar cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX41 antibody [EPR14298] (ab182007)
    Immunocytochemistry/ Immunofluorescence - Anti-DDX41 antibody [EPR14298] (ab182007)

    Immunofluorescence analysis of HeLa cells (fixative -20℃ Acetone) labeling DDX41 with ab182007 at a 1/250 dilution. Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX41 antibody [EPR14298] (ab182007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX41 antibody [EPR14298] (ab182007)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX41 with ab182007 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Western blot - Anti-DDX41 antibody [EPR14298] (ab182007)
    Western blot - Anti-DDX41 antibody [EPR14298] (ab182007)
    All lanes : Anti-DDX41 antibody [EPR14298] (ab182007) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 70 kDa

  • Anti-DDX41 antibody [EPR14298] (ab182007)
    Anti-DDX41 antibody [EPR14298] (ab182007)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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