Anti-DDX41 antibody (ab264173)
Key features and details
- Rabbit polyclonal to DDX41
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DDX41 antibody
See all DDX41 primary antibodies -
Description
Rabbit polyclonal to DDX41 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human DDX41 aa 572-622 (C terminal). The exact sequence is proprietary.
Database link: Q9UJV9 -
Positive control
- WB: HeLa and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDX41 antibody (ab264173)All lanes : Anti-DDX41 antibody (ab264173) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : NIH/3T3 whole cell lysate at 50 µg
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 3 minutes4-8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-DDX41 antibody (ab264173)DDX41 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264173 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264173 at 0.4 µg/ml.
Lane 1: ab264173 IP in HeLa whole cell lysate.
Lane 2: ab264173 IP in HeLa whole cell lysate.
Lane 3: Control IgG.Exposure time: 30 secs.
Different lots were used in lane 1 and lane 2.
Cells prepared using NETN lysis buffer.
