Anti-DDB1 antibody - C-terminal (ab226968)
Key features and details
- Rabbit polyclonal to DDB1 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DDB1 antibody - C-terminal
See all DDB1 primary antibodies -
Description
Rabbit polyclonal to DDB1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis
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Immunogen
Synthetic peptide within Human DDB1 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q16531 -
Positive control
- WB: A549, H1299, HEK-293T, Neuro-2a, GL261, C8D30, PC-12, HCT 116 and MCF7 whole cell extracts. ICC/IF: HeLa cells. IHC-P: U373 xenograft tissue; mouse heart, hind brain and duodenum tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDB1 antibody - C-terminal (ab226968)All lanes : Anti-DDB1 antibody - C-terminal (ab226968) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extracts
Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell extracts
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extracts
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 127 kDa7.5% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody - C-terminal (ab226968)Paraffin embedded mouse duodenum tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody - C-terminal (ab226968)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DDB1 (green) using ab226968 (1/500 dilution) in ICC/IF. HeLa cells were fixed in ice-cold methanol for 5 minutes. Counterstain: Hoechst 33343 (blue).
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Western blot - Anti-DDB1 antibody - C-terminal (ab226968)All lanes : Anti-DDB1 antibody - C-terminal (ab226968) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : DDB1 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 127 kDa7.5% SDS-PAGE
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Western blot - Anti-DDB1 antibody - C-terminal (ab226968)All lanes : Anti-DDB1 antibody - C-terminal (ab226968) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
Lane 2 : GL261 whole cell extracts
Lane 3 : C8D30 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 127 kDa7.5% SDS PAGE
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Western blot - Anti-DDB1 antibody - C-terminal (ab226968)Anti-DDB1 antibody - C-terminal (ab226968) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 127 kDa7.5% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody - C-terminal (ab226968)Paraffin embedded mouse hind brain tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody - C-terminal (ab226968)Paraffin embedded mouse heart tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody - C-terminal (ab226968)Paraffin embedded U373 (human) xenograft tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.
