Anti-DDB1 antibody (ab97522)
Key features and details
- Rabbit polyclonal to DDB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DDB1 antibody
See all DDB1 primary antibodies -
Description
Rabbit polyclonal to DDB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human DDB1 aa 749-1123.
Database link: Q16531 -
Positive control
- WB: NIH/3T3, PC-12, Rat2, HEK-293T, A431, HeLa and HepG2 whole cell lysate. IHC-P: Cal27 Xenograft, rat fore brain tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft , using ab97522 at 1/500 dilution.
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All lanes : Anti-DDB1 antibody (ab97522) at 1/1000 dilution
Lane 1 : PC-12 whole cell extracts
Lane 2 : Rat2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 127 kDa5% SDS-PAGE
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ab97522 at 1/200 dilution staining DDB1 in Paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe.
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Anti-DDB1 antibody (ab97522) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 127 kDa5% SDS PAGE
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Paraffin embedded mouse prostate tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.
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Anti-DDB1 antibody (ab97522) at 1/1000 dilution + NIH/3T3 whole cell lysate at 30 µg
Predicted band size: 127 kDa
7.5% SDS-PAGE. -
All lanes : Anti-DDB1 antibody (ab97522) at 1/1000 dilution
Lane 1 : HEK-293T whole cell extract
Lane 2 : A431 whole cell extract
Lane 3 : HeLa whole cell extract
Lane 4 : HepG2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 127 kDa5% SDS-PAGE
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Paraffin embedded rat fore brain tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.