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Anti-DDB1 antibody (ab97522)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-DDB1 antibody (ab97522)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DDB1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DDB1 antibody
    See all DDB1 primary antibodies
  • Description

    Rabbit polyclonal to DDB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human DDB1 aa 749-1123.
    Database link: Q16531

  • Positive control

    • WB: NIH/3T3, PC-12, Rat2, HEK-293T, A431, HeLa and HepG2 whole cell lysate. IHC-P: Cal27 Xenograft, rat fore brain tissue. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)

    Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft , using ab97522 at 1/500 dilution.

  • Western blot - Anti-DDB1 antibody (ab97522)
    Western blot - Anti-DDB1 antibody (ab97522)
    All lanes : Anti-DDB1 antibody (ab97522) at 1/1000 dilution

    Lane 1 : PC-12 whole cell extracts
    Lane 2 : Rat2 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 127 kDa



    5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody (ab97522)
    Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody (ab97522)
    ab97522 at 1/200 dilution staining DDB1 in Paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe.
  • Western blot - Anti-DDB1 antibody (ab97522)
    Western blot - Anti-DDB1 antibody (ab97522)
    Anti-DDB1 antibody (ab97522) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 127 kDa



    5% SDS PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)

    Paraffin embedded mouse prostate tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.

  • Western blot - Anti-DDB1 antibody (ab97522)
    Western blot - Anti-DDB1 antibody (ab97522)
    Anti-DDB1 antibody (ab97522) at 1/1000 dilution + NIH/3T3 whole cell lysate at 30 µg

    Predicted band size: 127 kDa



    7.5% SDS-PAGE.
  • Western blot - Anti-DDB1 antibody (ab97522)
    Western blot - Anti-DDB1 antibody (ab97522)
    All lanes : Anti-DDB1 antibody (ab97522) at 1/1000 dilution

    Lane 1 : HEK-293T whole cell extract
    Lane 2 : A431 whole cell extract
    Lane 3 : HeLa whole cell extract
    Lane 4 : HepG2 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 127 kDa



    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)

    Paraffin embedded rat fore brain tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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