Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5588] to DC-SIGN - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free
    See all DC-SIGN primary antibodies

  • Description

    Rabbit monoclonal [EPR5588] to DC-SIGN - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HACAT, fetal skin, fetal artery and Human small intestine lysates.

  • General notes

    Ab210401 is the carrier-free version of ab124828. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab210401 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)
    Western blot - Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)
    Anti-DC-SIGN antibody [EPR5588] (ab124828) at 1/1000 dilution (purified) + Human skin tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 46 kDa



    This data was developed using ab124828, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)
    Western blot - Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)
    All lanes : Anti-DC-SIGN antibody [EPR5588] (ab124828) at 1/1000 dilution (unpurified)

    Lane 1 : HACAT cell lysate
    Lane 2 : Fetal skin tissue lysate
    Lane 3 : Fetal artery tissue lysate
    Lane 4 : Human small intestine lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab124828, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)
    OI-RD Scanning - Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)
    This data was developed using ab124828, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)
    Anti-DC-SIGN antibody [EPR5588] - BSA and Azide free (ab210401)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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