Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10932-62] to Cytokeratin 9
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 9 antibody [EPR10932-62]
    See all Cytokeratin 9 primary antibodies

  • Description

    Rabbit monoclonal [EPR10932-62] to Cytokeratin 9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Cytokeratin 9. The exact sequence is proprietary.
    Database link: P35527

  • Positive control

    • NCI-H460, HL60, A431 and HeLa whole cell lysate (ab150035); Human skin tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10932-62

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    All lanes : Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966) at 1/1000 dilution

    Lane 1 : NCI-H460 cell lysate
    Lane 2 : HL60 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue, labeling Cytokeratin 9 using ab171966 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

    ab171966 showing +ve staining in Human skin tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

    ab171966 showing -ve staining in Human spleen tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

    ab171966 showing -ve staining in Human normal uterus tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

    ab171966 showing -ve staining in Human thyroid gland carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

    ab171966 showing -ve staining in Human colon adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling Cytokeratin 9, using ab171966 at 1/10 dilution.

  • Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
    Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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