Anti-Cytokeratin 9 antibody [EPR10932] (ab167398)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10932] to Cytokeratin 9
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Cytokeratin 9 antibody [EPR10932]
See all Cytokeratin 9 primary antibodies -
Description
Rabbit monoclonal [EPR10932] to Cytokeratin 9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment corresponding to Human Cytokeratin 9.
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Positive control
- WB: NCI-H460, A431, HeLa, and HL-60 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10932 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cytokeratin 9 antibody [EPR10932] (ab167398) at 1/10000 dilution
Lane 1 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDaBlocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBSTExposure time:
Lane 1 to 3: 30 seconds
Lane 4: 180 seconds -